描述

Fluorescence Resonance Energy Transfer(FRET) can be used to study protein-protein interactions in living cells. Protein interactions are critical formany processes inmammalian cells. Such interactions include the stable association of proteins within multi-subunit complexes and the transient association of regulatory proteins. Information about protein interactions in cells has previously come from either in vitro analyses using recombinant expressed proteins, or from yeast 2–hybrid studies. A limitation of this approach is that the protein interaction is studied in isolation, without regard to the many competing protein interactions that can occur within cells.

详细说明

出品公司：Addgene

载体类型：蛋白检测系统

启动子：CMV

关键功能：FRET检测

原核抗性：Kan

真核抗性：Neomycin