载体介绍:

pACYC184是一种含有p15A复制起点的大肠杆菌质粒克隆载体。这允许pACYC184在与ColE1相容性组的质粒（例如pBR322，pUC19）的细胞中共存。它是一个低拷贝数载体，每个细胞约15个拷贝，但可以用壮观霉素放大。由于cat基因的存在，氯霉素不能用于提高质粒产量（阻碍菌体复制，不阻碍质粒扩增）。

产品参数：

质粒类型：大肠杆菌克隆载体

出品公司：NEB

高拷贝/低拷贝：低拷贝

克隆方法：多克隆位点，限制性内切酶

载体大小：4245bp 

载体抗性：Chloramphenicol(氯霉素)

筛选标记：Tetracycline (四环素)

Propagation in E.coli
（1） 克隆菌株：DH5α、TOP10、XL1-Blue 等均可。
（2） 原核抗性：Chl+（Chloramphenicol，氯霉素）、Tet+（Tetracycline，四环素）
（3） 培养温度：37℃。

pACYC184 is an E. coli plasmid cloning vector containing the p15A origin of replication. This allows pACYC184 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell, but can be amplifed with spectinomycin. Chloramphenicol cannot be used for amplifcation due to the presence of the cat gene.

pACYC184 can be used together with, or as an alternative multipurpose vector to, pBR322. The origin of replication of pACYC184 is derived from the miniplasmid p15A1, whereas that of pBR322 and its derivatives is from pColE. These origins are compatible and pACYC184 and pBR322 can therefore co-exist within the same cell. This allows two recombinant plasmids to be maintained and expressed simultaneously.