描述：

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming the specificity of the CRISPR-mediated nucleic acid cleavage. Using lentivirus, we delivered the type II CRISPR nuclease system to facilitate genome editing in mammalian cells in a pooled library, targeting 18,080 genes with 64,751 sgRNAs in early consecutive exons.

基础信息：

载体类型：基因敲除载体

 启动子：EFS

出品公司：Addgene

关键功能：Unique sgRNA

原核抗性：Amp

真核检测：Puromycin