载体简介:
Positive control plasmid. In the trans-reporting system use with the c-Jun reporting system. In the cis-reporting system use with pNF-kB-Luc, pNF-kB-hrGFP, pAP1-Luc, pAP1-hrGFP, and pSRE-Luc plasmids.
The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.
Identify pathway-specific transcription activation
Easier, faster than Western blotting or gel-shift assays
Sensitive, quantitative assays
Antibodies now available
Stable cell lines available for constitutive expression of CREB, Elk1, c-Jun, and CHOP
PathDetect Trans-Reporting Systems -Identify specific stimuli that leads to the phosphorylation of a test transcription factor
产品参数:
载体名称:pFC-MEKK
出品公司:Stratagene
质粒类型:信号通路报告载体
克隆方法:多克隆位点,限制性内切酶