产品简介：

pTet-On expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. pTet-On can be distinguished from pTet-Off by digestion with Hind III.

 

产品参数：

质粒类型：四环素调控系统

出品公司：Clontech

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：7400 bp 

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neomycin (新霉素)