载体介绍：

 

pLPCX contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression (1–3). Upon transfection into a packaging cell line, pLPCX transiently expresses, or integrates and stably expresses, a transcript containing Ψ+ (the extended viral packaging signal) the puromycin resistance gene, and the gene of interest. The 5' viral LTR in this vector contains promoter/enhancer sequences that control expression of the puromycin resistance (Puror) gene for antibiotic selection in eukaryotic cells. A gene of interest can be cloned into the multiple cloning site immediately downstream of the human cytomegalovirus (CMV) immediate early promoter (PCMV IE). pLPCX also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.

 

产品参数：

 

质粒类型:逆转录病毒载体

 

启动子:CMV

 

载体大小:6265 bp

 

5' 测序引物及序列:LNCX: 5'd[AGCTCGTTTAGTGAACCGTCAGATC]3'

 

载体抗性:Ampicillin (氨苄青霉素)

 

筛选标记:Puromycin (嘌呤霉素)