载体介绍：

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:

The OpIE2 promoter for constitutive expression

The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein

The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks

C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin

产品参数：

出品公司：Invitrogen

质粒类型：昆虫细胞表达载体

高拷贝/低拷贝：高拷贝

启动子：OpIE2

克隆方法：多克隆位点，限制性内切酶

载体大小：3600bp

5' 测序引物及序列：OpIE2-F:5'd[CGCAACGATCTGGTAAACAC]3'

载体标签：HBM (Nterm), V5 (Cterm), His (Cterm)

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Blasticidin

备注：在昆虫细胞中能够稳定表达，HBM标签可实现目的蛋白的分泌表达

质粒图谱：