质粒简介:
pLKO.1-Puro is a cloning vector of shRNA, originally derived from HIV-1. It contains all the necessary cis-elements for packaging, reverse transcription, and integration for subsequent production of the lentiviral particles.
产品参数:
质粒名称:pLKO.1-Puro
出品公司:Addgene
启动子:hPGK,U6,T7,T3
复制子:SV40 Ori,F1 Ori
终止子:SV40 poly(A) signal,
质粒分类:病毒系列,慢病毒干扰载体
质粒大小:7032bp
原核抗性:Amp
克隆菌株:Stbl3
培养条件:37℃,有氧 LB
表达宿主:哺乳细胞
诱导方式:无须诱导,瞬时表达
5'测序引物:U6:ATGGACTATCATATGCTTACCGTA
3'测序引物:根据序列设计引物