质粒简介：

This manual provides details and information necessary to generate expression constructs of your gene of interest in the pCDH cDNA Cloning and Expression Lentivectors. Specifically, it provides critical instructions on amplification and cloning cDNA into the pCDH vectors, and verification of the final expression constructs. This manual does not include information on packaging the pCDH expression constructs into pseudotyped viral particles or transducing your target cells of choice with these particles. This information is available in the user manual Lentivector Expression Systems: Guide to Packaging and Transduction of Target Cells which is available on the SBI website. Before using the reagents and material supplied with this system, please read the entire manual.

产品参数：

载体名称:pCDH-MCS-T2A-Puro-MSCV

出品公司：SBI

质粒类型:慢病毒表达载体；cDNA表达载体

克隆方法:多克隆位点，限制性内切酶

启动子:MSCV

载体大小:--

5' 测序引物及序列:5’-GGGGTACAGTGCAGGGGAAAGAAT-3’

3' 测序引物及序列:--

载体标签:无

载体抗性:氨苄青霉素（Ampicillin）

筛选标记:嘌呤霉素

克隆菌株:E.coli   cells(RecA-)推荐:   Stbl2 ,OmniMAX 2 T1R

宿主细胞（系）:造血干细胞和胚胎干细胞

备注:pCDH-MCS-T2A-Puro-MSCV慢病毒表达载体是基于HIV的慢病毒载体；用于cDNA表达和克隆；高效转染细胞，建立稳定细胞系，表达水平高；单一启动子，由MSCV启动子驱动目 的基因和下游报告基因转录为双顺反子；二者之间含一段T2A肽，能够在翻译后水平上自我剪切，保证目的蛋白与荧光蛋白同等水平翻译。

稳定性:稳表达

组成型/诱导型:组成型

病毒/非病毒:慢病毒（HIV)