质粒简介:
Most cell types (split and non split cells); primary cell differentiation pCDH-EF1-MCS-T2A-Puro lentiviral expression vector of lentiviral vector is based on HIV for cDNA; cloning and expression; transfection efficiency of cells, to establish a stable cell line; single promoter driven by EF1a promoter and gene transcription and resistance genes for bicistron; between the two with a T2A peptide to shear on the translation level on the self, to ensure that the same genes and resistance gene translation level.
产品参数:
产品名称:pCDH-EF1-MCS-T2A-Puro
出品公司:SBI
复制子: pUC ori,SV40 ori
终止子: SV40 poly(A) signal
质粒分类: 病毒系列,慢病毒克隆载体
质粒大小: 7087bp
原核抗性: Amp
筛选标记: Puro
克隆菌株: Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: 根据序列设计引物
3'测序引物: 根据序列设计引物