载体介绍:
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag. • Strong polyhedrin (PH) promoter for high-level protein expression. • N-terminal 6xHis tag for purification of recombinant fusion proteins using metal-chelating resin and a TEV protease cleavage site for removal of the 6xHis tagfollowing protein purification. Vector supplied in 3 reading frames for simplified cloning.
产品参数:
出品公司:Invitrogen质粒类型:昆虫细胞表达载体
启动子:Polyhedrin promoter
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:4858 bp
5' 测序引物:pFastbac-F:5'-TATTCCGGATTATTCATACC-3'
3' 测序引物:pFastBac-R:5'-ACAAATGTGGTATGGCTGA-3'
载体标签:N-His, N-TEV
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Gentamicin(庆大霉素)
质粒图谱: