产品简介:

p53-AbAi is a yeast reporter vector that serves as a positive control in the Matchmaker Gold Yeast One-Hybrid Library Screening System. The vector contains a p53 binding site, located upstream of the yeast iso-1-cytochrome C minimal promoter and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A. Expression of AUR1-C, and thus AbA resistance, is induced by the binding of GAL4 activation domain-p53 fusion proteins to the p53 binding site upstream of AUR1-C. p53-AbAi cannot be propagated episomally in yeast; it can only be stably maintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the nonfunctional ura3-52 locus of the yeast strain provided in the Matchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for the selection of recombinant yeast.

 

产品参数：

质粒类型：酵母系列，酵母单杂交系统

出品公司：Clontech

载体大小：4944 bp

载体抗性：Ampicillin

筛选标记：URA3

复制子：pUC ori

克隆菌株：DH5α

培养条件：37℃，有氧 LB

表达宿主：酵母细胞

5'测序引物：根据序列设计引物

3'测序引物：根据序列设计引物