质粒简介:
When used with any Lenti-X Tet-On 3G or Tet-Express inducible expression system, the pLVX-TRE3G Vector Set allows for lentiviral delivery and inducible expression of your gene of interest in a wide variety of mammalian cells. Target cells that contain a gene of interest under the control of a PTRE3GV promoter will express high levels of your gene of interest, when cultured in the presence of doxycycline (when used with the pLVX-Tet3G Vector and a Tet-On 3G system) or in the presence of the Tet-Express tetracycline transactivator (when used with Tet-Express Systems).
产品参数:
启动子: TRE3GV,PGK
复制子: pUC ori
终止子: SV40 poly(A) signal
质粒分类: 病毒系列,慢病毒克隆载体
质粒大小: 7819bp
原核抗性: Amp
筛选标记: Puro
克隆菌株: Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 四环素诱导
5'测序引物: M13R:CAGGAAACAGCTATGACC
3'测序引物: 根据序列设计引物