pLVX-IRES-mCherry is an HIV-1-based, lentiviral expression vector that allows the simultaneous expression 

of your protein of interest and mCherry in virtually any mammalian cell type, including primary cells. mCherry 

is a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed (1). 

The vector expresses the two proteins from a bicistronic mRNA transcript, allowing mCherry to be used as an 

indicator of transduction efficiency and a marker for selection by flow cytometry.

 

Expression of the bicistronic transcript is driven by the constitutively active human cytomegalovirus immediate

 early promoter (PCMV IE) located just upstream of the MCS. An encephalomyocarditis virus (EMCV) internal 

ribosome entry site (IRES), positioned between the MCS and mCherry, facilitates cap-independent translation

 of mCherry from an internal start site at the IRES/mCherry junction (1).

 

pLVX-IRES-mCherry contains all of the viral processing elements necessary for the production of 

replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, 

and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element

 (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA (2), leading to 

increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), 

which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (3). 

Finally, pLVX-IRES-mCherry also contains a central polypurine tract/central termination sequence element

 (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import

 of the viral genome, resulting in improved vector integration and more efficient transduction (4). 

The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) 

for propagation and selection in bacteria.

 

产品参数：

 

启动子: CMV IE

 

复制子: pUC ori

 

质粒分类: 病毒系列，慢病毒克隆载体

 

质粒大小: 8172bp

 

原核抗性: Amp

 

克隆菌株: Stbl3

 

培养条件: 37℃，有氧 LB

 

表达宿主: 哺乳细胞

 

诱导方式: 无须诱导，瞬时表达

 

5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG

 

3'测序引物: 根据序列设计引物