产品简介：

pAbAi is a yeast reporter vector that can be used in one-hybrid assays to identify and characterize DNA-binding proteins. The vector, which was specifically designed for use with the Matchmaker™ Gold Yeast One-Hybrid Library Screening System (Cat. No. 630491), contains a multiple cloning site (MCS) upstream of the yeast iso-1-cytochrome C minimal promoter(not shown) and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A (AbA; Cat. No. 630466). A cis-acting element (i.e., a target sequence) can be inserted into the MCS and used as bait to screen GAL4 AD/cDNA fusion libraries (e.g. Mate & Plate™ Libraries) for proteins that interact with the target sequence. Positive protein-DNA (i.e.,one-hybrid) interactions drive the expression of AUR1-C. As a result, one-hybrid interactions can be detected by selecting for yeast that are resistant to AbA.

pAbAi cannot be propagated episomally in yeast; it can only be stably maintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the ura3-52 locus of the yeast strain provided in theMatchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for selection of recombinant yeast (1, 2). The vector also contains a Col E1 origin of replication and an ampicillin resistance gene (Ampr) for propagation and selection in E.coli.

 

产品参数：

质粒类型：酵母单杂交系统

出品公司：Clontech

载体大小：4894 bp

载体抗性：Ampicillin

筛选标记：URA3

克隆方法： 多克隆位点，限制性内切酶

启动子： ADH1

5' 测序引物及序列： T7： TAATACGACTCACTATAGGG