产品简介：

The Flp-In System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location.The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice. An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase-mediated DNA recombination at the FRT site. A Flp-In target site vector,pFRT/lacZeo,for generation of a host cell line containing an integrated FRT site.Integration of the pFRT/lacZeo plasmid into the genome is random.

pFRT/lacZeo and pFRT/lacZeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a: 

• FRT site for subsequent targeted integration of a Flp-In™ expression vector

• Z-Zeocin™ fusion for selection of stable integrants with Zeocin™

These vectors differ in the SV40 promoter that drives expression of the lacZ-Zeocin™ fusion. pFRT/lacZeo2 contains a truncated version of the SV40 promoter. Use of this vector facilitates the isolation of clones that have integrated the vector near enhancer elements in the genome. Selection of stable clones using pFRT/lacZeo2 may allow easier isolation of clones that express a gene of interest at higher levels.

 

产品参数：

质粒类型：FLP/FRT位点特异性重组系统

出品公司：Invitrogen

启动子：SV40

载体大小：8106 bp

载体抗性：Ampicillin

筛选标记：Zeocin