产品简介：

Drosophila S2 cells may be transfected with the recombinant expression vector alone to assay for transient expression of the protein of interest or in combination with pCoBlast to generate stable cell lines. We recommend that you test for expression of your recombinant protein by transient transfection before undertaking selection for stable cell lines. The method of choice for transfection of S2 cells is calcium phosphate. Stable cell lines expressing heterologous proteins can be generated in 2 weeks following cotransfection of the expression vector and the pCoBlast selection vector. By optimizing the ratio of expression vector to selection vector, cell lines with a very high copy number of the desired gene can be generated, leading to increased expression levels of the desired protein.

 

产品参数：

载体类型：果蝇细胞表达载体

出品公司：Invitrogen

启动子：copia

载体大小：3907 bp

关键功能：提供筛选标记

原核抗性：Amp

真核抗性：Blasticidin

克隆菌株：TOP10；DH5-T1R

宿主细胞（系）：果蝇细胞系S2

备注：pCoBlast载体与昆虫表达载体共转染，建立稳表达细胞系。

组成型/诱导型：组成型