产品简介：

The DES-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:

• Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency

• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody

• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.

 

产品参数：

质粒类型：昆虫细胞表达载体

出品公司：Invitrogen

启动子：MT

载体大小：3642 bp

载体标签：V5 Epitope Tag, His Tag (6x)

载体抗性：Ampicillin (氨苄青霉素)