产品简介:
SUMO Protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae.
It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amino acid sequence.
The SUMO Protease features:
Highly active cleavage
No non-specific proteolysis (highly specific cleavage*)
Activity from 2°C to 37°C
A six-histidine sequence to facilitate its removal from the digested protein sample
Application: Removal of fusion tags from recombinant proteins.Unit Definition: One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2µg of substrate protein at 30°C in one hour.
Quality Control: SUMO Protease has greater than 85% single-band purity with no non-specific protease contamination. It is functionally tested for the absence of any non-specific protease activity.
产品参数:
质粒类型:酵母表达载体
克隆方法:多克隆位点,限制性内切酶
载体标签:6XHis
载体抗性:卡纳霉素(Kanamycin)
备注:表达SUMO酶