Basic product information:
pAAV-IRES-hrGFP 载体
载体类型: 腺相关病毒系统
表达水平: 高
启动子: CMV
载体大小: 6.1 kb
3‘ 端测序引物: Beta-globin-F：ATTCTGAGTCCAAGCTAGGC
5‘ 端测序引物: hGH-PA-R: CCAGCTTGGTTCCCAATAGA
载体抗性: Ampicillin (氨苄青霉素) 

载体描述
The vector pAAV-IRES-hrGFP contains a dicistronic expression cassette in which the Renilla reniformis green fluorescent protein (hrGFP) gene is expressed as a second open reading frame downstream of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES). The hrGFP gene serves as a useful marker for titering the virus and ascertaining the infection efficiency for the desired target cell type. Another unique feature of this vector is the FLAG sequence (3x) at the c-terminal of MCS. The foreign gene could be cloned in frame with the tag and the protein could be further purified or detected by anti-FLAG antibody.

注意事项：
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解，在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用，菌体会逐渐沉淀至管底，请混匀后使用。随着冻融次数的增加，菌株的活力会逐渐下降。