Model key characteristics of the adult intestinal epithelium using intestinal organoids established and maintained

 

with this complete medium formulation and optimized protocol. Using the easy-to-follow and robust protocol, you can

 

derive organoids from human intestinal crypts in one week; organoid growth across donor samples, including those

 

that are otherwise difficult to grow, is enabled by an enriched stem cell population.

 

Organoids grown in IntestiCult™ Organoid Growth Medium (Human) incorporate a functional lumen enclosed by a polarized

 

intestinal epithelial cell layer and, for versatile modeling applications, can be further differentiated in 3D or in 2D as submerged

 

monolayers or air-liquid interface (ALI) cultures using IntestiCult™ Organoid Differentiation Medium (Human; Catalog #100-0214).

 

Applications of intestinal organoid cultures include studying the development and function of intestinal epithelium, modeling intestinal

 

diseases, and screening molecules for both efficacy and toxicity in an intestinal model. Intestinal organoid cultures can also be used for

 

investigation of adult stem cell properties and for regenerative therapy approaches.

 

Learn how to culture human intestinal organoids in our On-Demand Intestinal Course or browse our Frequently Asked Questions (FAQs)

 

about the organoid workflows using IntestiCult™.

 

Should you intend to use this product for commercial purposes, please contact HUB at www.huborganoids.nl for a commercial use

license or for clarifications in relation to HUB licensing.

SUBTYPE:专业培养基

CELL TYPE:肠细胞

SPECIES:人

APPLICATION:分化, 扩增, 类器官培养, 细胞培养, 维持培养

BRAND:IntestiCult

AREA OF INTEREST:上皮细胞生物学, 干细胞生物学, 疾病模型, 药物发现和毒性测试