Target：PI3 Kinase P85α
Fields：EGFR tyrosine kinase inhibitor resistance;Endocrine resistance;Platinum drug resistance;ErbB signaling pathway;Ras signaling pathway;Rap1 signaling pathway;cAMP signaling pathway;Chemokine signaling pathway;HIF-1 signaling pathway;FoxO signaling pathway;Phosphatidylinositol signaling system;Sphingolipid signaling pathway;Phospholipase D signaling pathway;Autophagy - animal;mTOR signaling pathway;PI3K-Akt signaling pathway;AMPK signaling pathway;Apoptosis;Longevity regulating pathway;Longevity regulating pathway - multiple species;Cellular senescence;Axon guidance;VEGF signaling pathway;Osteoclast differentiation;Focal adhesion;Signaling pathways regulating pluripotency of stem cells;Platelet activation;Neutrophil extracellular trap formation;Toll-like receptor signaling pathway;C-type lectin receptor signaling pathway;JAK-STAT signaling pathway;Natural killer cell mediated cytotoxicity;T cell receptor signaling pathway;>
Gene Name：PIK3R1
Protein Name：Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3-kinase regulatory subunit alpha) (PI3K regulatory subunit alpha) (PtdIns-3-kinase regulatory subunit alpha) (Phosphatidylinositol 3-kinase 8
Human Gene Id：5295
Human Swiss Prot No：P27986
Mouse Swiss Prot No：P26450
Rat Swiss Prot No：Q63787
Immunogen：Recombinant Protein of PI3 Kinase P85α
Specificity：PI3 Kinase P85α protein detects endogenous levels of PI3 Kinase P85α
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Monoclonal, Mouse
Dilution：WB 1:1000-2000, IHC 1:100-200. IF 1:50-200
Purification：The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：PIK3R1;GRB1;Phosphatidylinositol 3-kinase regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase subunit p85-alpha;PtdIns-3-kinase regulatory subunit p85-alpha
Observed Band(KD)：85kD
Background： Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],
Function：disease:Defects in PIK3R1 are a cause of severe insulin resistance.,domain:The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.,function:Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.,PTM:Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.,similarity:Belongs to the PI3K p85 subunit family.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 SH2 domains.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat
Subcellular Location：nucleus,cytoplasm,cis-Golgi network,cytosol,plasma membrane,cell-cell junction,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,membrane,perinuclear endoplasmic reticulum membrane,
Expression：Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
