Target：HIF-1β

Fields：HIF-1 signaling pathway;Cushing syndrome;Pathways in cancer;Chemical carcinogenesis - receptor activation;Chemical carcinogenesis - reactive oxygen species;Renal cell carcinoma

Gene Name：ARNT

Protein Name：Aryl hydrocarbon receptor nuclear translocator (ARNT protein) (Class E basic helix-loop-helix protein 2) (bHLHe2) (Dioxin receptor, nuclear translocator) (Hypoxia-inducible factor 1-beta) (HIF-1-beta)

Human Gene Id：405

Human Swiss Prot No：P27540

Mouse Swiss Prot No：P53762

Immunogen：Recombinant Protein of HIF-1 β

Specificity：HIF-1 β/ARNT protein detects endogenous levels of HIF-1 β/ARNT

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Monoclonal, Mouse

Dilution：WB 1:1000-2000, IHC 1:100-200, IF 1:50-200

Purification：The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ARNT;BHLHE2;Aryl hydrocarbon receptor nuclear translocator;ARNT protein;Class E basic helix-loop-helix protein 2;bHLHe2;Dioxin receptor, nuclear translocator;Hypoxia-inducible factor 1-beta;HIF-1-beta;HIF1-beta

Observed Band(KD)：87kD

Background： This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013],

Function：function:Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. Interacts with TACC3 (By similarity). Interacts with NOCA7.,

Subcellular Location：Nucleus.

Expression： Aorta endothelial cell,Brain,Kidney,Thalamus,Uterus,