Target：LC3B
Fields：Mitophagy - animal;Autophagy - animal;Ferroptosis;Apelin signaling pathway;NOD-like receptor signaling pathway;Amyotrophic lateral sclerosis;Pathways of neurodegeneration - multiple diseases;Shigellosis;Kaposi sarcoma-associated herpesvirus infection
Gene Name：MAP1LC3B
Protein Name：MAP1LC3B
Human Gene Id：81631
Human Swiss Prot No：Q9GZQ8
Mouse Swiss Prot No：Q9CQV6
Immunogen：Recombinant Protein of LC3B of MAP1LC3B
Specificity：LC3B protein detects endogenous levels of MAP1LC3B
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Monoclonal, Mouse
Dilution：WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000. IF 1:50-200
Purification：The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：MAP1LC3B
Observed Band(KD)：14,16kD
Background： The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],
Function：caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,
Subcellular Location：Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .
Expression：Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
