His-Tag Mouse mAb
Cat. QYA04393A
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation,
and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. A variety of plasmids contain DNA that encodes an aminoterminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.
Source
The antibody was affinity-purified by affinity-chromatography using specific immunogen.
Product
Each vial contains 100ug mouse IgG diluted in 100ul of PBS pH7.4 containing 0.02% sodium azide and 50% glycerol. The antibody concentration is 1mg/ml.
Specificity
The antibody detects C-terminal, internal, and N-terminal his-tag fusion proteins.
Applications and Suggested Working Concentration
WB: 1:5000-1:10000
Storage
Storage at -20℃. Do not aliquot the antibody. Stable for one year from the date of shipment.
Research Use：For research use only, not for use in diagnostic procedures.
