Target：GRK 2
Fields：Chemokine signaling pathway;Endocytosis;Hedgehog signaling pathway;Glutamatergic synapse;Olfactory transduction;Morphine addiction
Gene Name：ADRBK1
Protein Name：Beta-adrenergic receptor kinase 1
Human Gene Id：156
Human Swiss Prot No：P25098
Mouse Swiss Prot No：Q99MK8
Rat Gene Id：25238
Rat Swiss Prot No：P26817
Immunogen：The antiserum was produced against synthesized peptide derived from human GRK2 around the phosphorylation site of Ser685. AA range:640-689
Specificity：Phospho-GRK 2 (S685) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S685.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：ADRBK1;BARK;BARK1;GRK2;Beta-adrenergic receptor kinase 1;Beta-ARK-1;G-protein coupled receptor kinase 2
Observed Band(KD)：80kD
Background： The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.,online information:Beta adrenergic receptor kinase entry,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,subunit:Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5.,tissue specificity:Expressed in peripheral blood leukocytes.,
Subcellular Location：Cytoplasm . Cell membrane . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .
Expression：Expressed in peripheral blood leukocytes.
