Target：Cdc25C
Fields：Cell cycle;Oocyte meiosis;Progesterone-mediated oocyte maturation;Human immunodeficiency virus 1 infection;MicroRNAs in cancer
Gene Name：CDC25C
Protein Name：M-phase inducer phosphatase 3
Human Gene Id：995
Human Swiss Prot No：P30307
Mouse Swiss Prot No：P48967
Immunogen：The antiserum was produced against synthesized peptide derived from human CDC25C around the phosphorylation site of Ser216. AA range:183-232
Specificity：Phospho-Cdc25C (S216) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at S216.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：CDC25C;M-phase inducer phosphatase 3;Dual specificity phosphatase Cdc25C
Observed Band(KD)：53kD
Background：cell division cycle 25C(CDC25C) Homo sapiens This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015],
Function：catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,developmental stage:Expressed predominantly in G2 phase.,function:Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity.,PTM:Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,subunit:Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity.,
Subcellular Location：Nucleus .
Expression： Colon carcinoma,Epithelium,Skin,Testis,
