Target：Bcr
Fields：Pathways in cancer;Chronic myeloid leukemia
Gene Name：BCR
Protein Name：Breakpoint cluster region protein
Human Gene Id：613
Human Swiss Prot No：P11274
Mouse Gene Id：110279
Mouse Swiss Prot No：Q6PAJ1
Immunogen：The antiserum was produced against synthesized peptide derived from human Bcr around the phosphorylation site of Tyr360. AA range:331-380
Specificity：Phospho-Bcr (Y360) Polyclonal Antibody detects endogenous levels of Bcr protein only when phosphorylated at Y360.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：BCR;BCR1;D22S11;Breakpoint cluster region protein;Renal carcinoma antigen NY-REN-26
Observed Band(KD)：142kD
Background： A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).,domain:The DH domain is involved in interaction with CCPG1.,domain:The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.,function:GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.,PTM:Autophosphorylated.,similarity:Contains 1 C2 domai
Subcellular Location：Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, axon . Cell junction, synapse .
Expression： Brain,Epithelium,Platelet,Renal cell carcinoma,T-cell,
