Target：A-Raf
Fields：EGFR tyrosine kinase inhibitor resistance;Endocrine resistance;MAPK signaling pathway;ErbB signaling pathway;FoxO signaling pathway;Vascular smooth muscle contraction;Natural killer cell mediated cytotoxicity;Long-term potentiation;Serotonergic synapse;Long-term depression;Regulation of actin cytoskeleton;Insulin signaling pathway;Progesterone-mediated oocyte maturation;Parathyroid hormone synthesis, secretion and action;Alzheimer disease;Pathways of neurodegeneration - multiple diseases;Alcoholism;Hepatitis C;Hepatitis B;Pathways in cancer;Proteoglycans in cancer;Chemical carcinogenesis - reactive oxygen species;Colorectal cancer;Renal cell carcinoma;Pancreatic cancer;Endometrial cancer;Glioma;Prostate cancer;Melanoma;Bladder cancer;Chronic myeloid leukemia;Acute myeloid leukemia;Non-small cell lung cancer;Breast cancer;Hepatocellular carcinoma;Gastric cancer
Gene Name：ARAF
Protein Name：Serine/threonine-protein kinase A-Raf
Human Gene Id：369
Human Swiss Prot No：P10398
Mouse Gene Id：11836
Mouse Swiss Prot No：P04627
Rat Gene Id：64363
Rat Swiss Prot No：P14056
Immunogen：The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Ser299. AA range:266-315
Specificity：Phospho-A-Raf (S299) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at S299.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：ARAF;ARAF1;PKS;PKS2;Serine/threonine-protein kinase A-Raf;Proto-oncogene A-Raf;Proto-oncogene A-Raf-1;Proto-oncogene Pks
Molecular Weight(Da)：68kD
Background： This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2 zinc ions per subunit.,function:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RBD (Ras-binding) domain.,subunit:Interacts with TH1L/NELFD.,tissue specificity:Predominantly in urogenital tissues.,
Subcellular Location：intracellular,mitochondrion,cytosol,
Expression：Predominantly in urogenital tissues.
