Target：Eps15

Fields：Endocytosis

Gene Name：EPS15

Protein Name：Epidermal growth factor receptor substrate 15

Human Gene Id：2060

Human Swiss Prot No：P42566

Mouse Gene Id：13858

Mouse Swiss Prot No：P42567

Immunogen：Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849)

Specificity：Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EPS15;AF1P;Epidermal growth factor receptor substrate 15;Protein Eps15;Protein AF-1p

Observed Band(KD)：100kD

Background： This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009],

Function：disease:A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with MLL/HRX. The result is a rogue activator protein.,domain:The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.,function:Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits.,PTM:Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.,similarity:Contains 2 EF-hand dom

Subcellular Location：Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes. .; [Isoform 2]: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with HGS on bilayered clathrin coats on endosomes.

Expression：Ubiquitously expressed.