Target：Ephrin-B1/2
Fields：Axon guidance
Gene Name：EFNB1/EFNB2
Protein Name：Ephrin-B1/Ephrin-B2
Human Gene Id：1947/1948
Human Swiss Prot No：P98172/P52799
Mouse Gene Id：13641/13642
Rat Swiss Prot No：P52796
Immunogen：The antiserum was produced against synthesized peptide derived from human EFNB1/2 around the phosphorylation site of Tyr330. AA range:284-333
Specificity：Phospho-Ephrin-B1/2 (Y330) Polyclonal Antibody detects endogenous levels of Ephrin-B1/2 protein only when phosphorylated at Y330.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：EFNB1;EFL3;EPLG2;LERK2;Ephrin-B1;EFL-3;ELK ligand;ELK-L;EPH-related receptor tyrosine kinase ligand 2;LERK-2;EFNB2;EPLG5;HTKL;LERK5;Ephrin-B2;EPH-related receptor tyrosine kinase ligand 5;LERK-5;HTK ligand;HTK-L
Observed Band(KD)：59kD
Background： The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008],
Function：disease:Defects in EFNB1 are a cause of craniofrontonasal syndrome (CFNS) [MIM:304110]; also known as craniofrontonasal dysplasia (CFND). CFNS is an X-linked inherited syndrome characterized by hypertelorism, coronal synostosis with brachycephaly, downslanting palpebral fissures, clefting of the nasal tip, joint anomalies, longitudinally grooved fingernails and other digital anomalies.,function:Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.,induction:By TNF-alpha.,PTM:Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.,similarity:Belongs to the ephrin family.,subunit:Interacts with GRIP1 and GRIP2.,tissue specificity:Heart, placenta, lung, liver, skeletal muscle, kidney, pancreas.,
Subcellular Location：Cell membrane ; Single-pass type I membrane protein . Membrane raft . May recruit GRIP1 and GRIP2 to membrane raft domains. .; [Ephrin-B1 C-terminal fragment]: Cell membrane ; Single-pass type I membrane protein .; [Ephrin-B1 intracellular domain]: Nucleus . Colocalizes with ZHX2 in the nucleus. .
Expression：Widely expressed (PubMed:8070404, PubMed:7973638). Detected in both neuronal and non-neuronal tissues (PubMed:8070404, PubMed:7973638). Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord (PubMed:7973638).
