Target：Emt
Fields：Chemokine signaling pathway;T cell receptor signaling pathway;Leukocyte transendothelial migration
Gene Name：ITK
Protein Name：Tyrosine-protein kinase ITK/TSK
Human Gene Id：3702
Human Swiss Prot No：Q08881
Mouse Gene Id：16428
Mouse Swiss Prot No：Q03526
Immunogen：The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. AA range:478-527
Specificity：Phospho-Emt (Y512) Polyclonal Antibody detects endogenous levels of Emt protein only when phosphorylated at Y512.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500-2000 ,IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：ITK;EMT;LYK;Tyrosine-protein kinase ITK/TSK;Interleukin-2-inducible T-cell kinase;IL-2-inducible T-cell kinase;Kinase EMT;T-cell-specific kinase;Tyrosine-protein kinase Lyk
Observed Band(KD)：72kD
Background： This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,function:Plays a role in T-cell proliferation and differentiation.,induction:By interleukin-2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.,tissue specificity:T-cell lines and natural killer cell lines.,
Subcellular Location：Cytoplasm . Nucleus . Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
Expression：T-cell lines and natural killer cell lines.
