Target：Adrenergic Receptor β2

Fields：Calcium signaling pathway;cGMP-PKG signaling pathway;cAMP signaling pathway;Neuroactive ligand-receptor interaction;Adrenergic signaling in cardiomyocytes;Regulation of lipolysis in adipocytes;Renin secretion;Salivary secretion;Chemical carcinogenesis - receptor activation

Gene Name：ADRB2

Protein Name：Beta-2 adrenergic receptor

Human Gene Id：154

Human Swiss Prot No：P07550

Mouse Swiss Prot No：P18762

Immunogen：The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor beta2 around the phosphorylation site of Ser346. AA range:321-370

Specificity：Phospho-AR-β2 (S346) Polyclonal Antibody detects endogenous levels of AR-β2 protein only when phosphorylated at S346.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：ADRB2;ADRB2R;B2AR;Beta-2 adrenergic receptor;Beta-2 adrenoreceptor;Beta-2 adrenoceptor

Observed Band(KD)：40kD

Background： This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],

Function：disease:Polymorphic forms of ADRB2 could impart some form of nocturnal asthma.,function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.,PTM:Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.,PTM:Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the recep

Subcellular Location：Cell membrane ; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). .

Expression： Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid,