Target：p62 Dok
Gene Name：DOK1
Protein Name：Docking protein 1
Human Gene Id：1796
Human Swiss Prot No：Q99704
Mouse Gene Id：13448
Mouse Swiss Prot No：P97465
Rat Gene Id：312477
Rat Swiss Prot No：Q4QQV2
Immunogen：The antiserum was produced against synthesized peptide derived from human p62 Dok around the phosphorylation site of Tyr362. AA range:329-378
Specificity：Phospho-Dok-1 (Y362) Polyclonal Antibody detects endogenous levels of Dok-1 protein only when phosphorylated at Y362.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：DOK1;Docking protein 1;Downstream of tyrosine kinase 1;p62(dok);pp62
Observed Band(KD)：55kD
Background：docking protein 1(DOK1) Homo sapiens The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016],
Function：domain:The PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.,PTM:Constitutively tyrosine-phosphorylated.,PTM:Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.,similarity:Belongs to the DOK family. Type A subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with ABL (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3.,tissue specificity:Expressed in pancreas, heart, leukocyte and spleen
Subcellular Location：[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm, perinuclear region.
Expression：Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
