Target：DAPK1
Fields：Autophagy - animal;Pathways in cancer;Bladder cancer
Gene Name：DAPK1
Protein Name：Death-associated protein kinase 1
Human Gene Id：1612
Human Swiss Prot No：P53355
Mouse Gene Id：69635
Mouse Swiss Prot No：Q80YE7
Immunogen：The antiserum was produced against synthesized peptide derived from human DAPK1 around the phosphorylation site of Ser308. AA range:274-323
Specificity：Phospho-DAPK1 (S308) Polyclonal Antibody detects endogenous levels of DAPK1 protein only when phosphorylated at S308.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：DAPK1;DAPK;Death-associated protein kinase 1;DAP kinase 1
Molecular Weight(Da)：160kD
Background： Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-308.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,induction:Up-regulated following treatment with interferon-gamma.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 10 ANK repeats.,subcellular location:Colocalizes with the actin filament system.,
Subcellular Location：[Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.
Expression：Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
