Target：cPLA2
Fields：Glycerophospholipid metabolism;Ether lipid metabolism;Arachidonic acid metabolism;Linoleic acid metabolism;alpha-Linolenic acid metabolism;Metabolic pathways;MAPK signaling pathway;Ras signaling pathway;Phospholipase D signaling pathway;Necroptosis;Vascular smooth muscle contraction;VEGF signaling pathway;Platelet activation;Fc epsilon RI signaling pathway;Fc gamma R-mediated phagocytosis;Glutamatergic synapse;Serotonergic synapse;Long-term depression;Inflammatory mediator regulation of TRP channels;GnRH signaling pathway;Ovarian steroidogenesis;Oxytocin signaling pathway;Choline metabolism in cancer
Gene Name：PLA2G4A
Protein Name：Cytosolic phospholipase A2
Human Gene Id：5321
Human Swiss Prot No：P47712
Mouse Gene Id：18783
Mouse Swiss Prot No：P47713
Rat Swiss Prot No：P50393
Immunogen：The antiserum was produced against synthesized peptide derived from human c-PLA2 around the phosphorylation site of Ser505. AA range:471-520
Specificity：Phospho-cPLA2 (S505) Polyclonal Antibody detects endogenous levels of cPLA2 protein only when phosphorylated at S505.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：PLA2G4A;CPLA2;PLA2G4;Cytosolic phospholipase A2;cPLA2;Phospholipase A2 group IVA
Observed Band(KD)：110kD
Background： This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015],
Function：catalytic activity:2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate.,catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,domain:The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca(2+).,function:Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.,PTM:Activated by phosphorylation at both Ser-505 and Ser-727.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to membrane ve
Subcellular Location：Cytoplasm . Golgi apparatus membrane . Nucleus envelope. Translocates to intracellular membranes in a calcium-dependent way. .
Expression：Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level) (PubMed:25102815).
