Target：IRAK-1
Fields：MAPK signaling pathway;NF-kappa B signaling pathway;Toll-like receptor signaling pathway;Neurotrophin signaling pathway;Alcoholic liver disease;Pathogenic Escherichia coli infection;Salmonella infection;Pertussis;Yersinia infection;Leishmaniasis;Chagas disease;Toxoplasmosis;Tuberculosis;Hepatitis B;Measles;Herpes simplex virus 1 infection;Epstein-Barr virus infection;Human immunodeficiency virus 1 infection;Coronavirus disease - COVID-19;Lipid and atherosclerosis
Gene Name：IRAK1
Protein Name：Interleukin-1 receptor-associated kinase 1
Human Gene Id：3654
Human Swiss Prot No：P51617
Mouse Gene Id：16179
Mouse Swiss Prot No：Q62406
Immunogen：The antiserum was produced against synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr100. AA range:66-115
Specificity：Phospho-IRAK-1 (T100) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T100.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：IRAK1;IRAK;Interleukin-1 receptor-associated kinase 1;IRAK-1
Observed Band(KD)：77kD
Background： This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.,PTM:Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:
Subcellular Location：Cytoplasm . Nucleus . Lipid droplet . Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). .
Expression：Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
