Target：ILK
Fields：PPAR signaling pathway;Axon guidance;Focal adhesion;Bacterial invasion of epithelial cells;Shigellosis;Endometrial cancer
Gene Name：ILK
Protein Name：Integrin-linked protein kinase
Human Gene Id：3611
Human Swiss Prot No：Q13418
Mouse Gene Id：16202
Mouse Swiss Prot No：O55222
Rat Gene Id：170922
Rat Swiss Prot No：Q99J82
Immunogen：The antiserum was produced against synthesized peptide derived from human ILK around the phosphorylation site of Ser246. AA range:212-261
Specificity：Phospho-ILK (S246) Polyclonal Antibody detects endogenous levels of ILK protein only when phosphorylated at S246.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：ILK;ILK1;ILK2;Integrin-linked protein kinase;59 kDa serine/threonine-protein kinase;ILK-1;ILK-2;p59ILK
Molecular Weight(Da)：51kD
Background： This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:A PH-like domain is involved in phosphatidylinositol phosphate binding.,enzyme regulation:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.,function:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also
Subcellular Location：Cell junction, focal adhesion . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere .
Expression：Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
