Target：NOS2/iNOS
Fields：Arginine biosynthesis;Arginine and proline metabolism;Metabolic pathways;Calcium signaling pathway;HIF-1 signaling pathway;Peroxisome;Apelin signaling pathway;Relaxin signaling pathway;Alzheimer disease;Amyotrophic lateral sclerosis;Pathways of neurodegeneration - multiple diseases;Pertussis;Leishmaniasis;Chagas disease;Toxoplasmosis;Amoebiasis;Tuberculosis;Pathways in cancer;Small cell lung cancer
Gene Name：NOS2, INOS
Protein Name：Nitric oxide synthase inducible
Human Gene Id：4843
Human Swiss Prot No：P35228
Mouse Gene Id：18126
Mouse Swiss Prot No：P29477
Rat Gene Id：24599
Rat Swiss Prot No：Q06518
Immunogen：The antiserum was produced against synthesized peptide derived from human iNOS around the phosphorylation site of Tyr151. AA range:117-166
Specificity：Phospho-NOS2 (Y151) Polyclonal Antibody detects endogenous levels of NOS2 protein only when phosphorylated at Y151.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：NOS2;NOS2A;Nitric oxide synthase; inducible;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;NOS type II;Peptidyl-cysteine S-nitrosylase NOS2
Molecular Weight(Da)：131kD
Background： Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],
Function：catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.,function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.,induction:By endotoxins and cytokines.,online information:Nitric oxide synthase entry,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subunit:Homodimer. Bin
Subcellular Location：Cytoplasm, cytosol . Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. .
Expression：Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes (PubMed:7504305).
