Target:NFAT3
Fields:cGMP-PKG signaling pathway;Cellular senescence;Wnt signaling pathway;Axon guidance;C-type lectin receptor signaling pathway;Oxytocin signaling pathway;Hepatitis B;Human cytomegalovirus infection;Human T-cell leukemia virus 1 infection;Kaposi sarcoma-associated herpesvirus infection;Human immunodeficiency virus 1 infection
Gene Name:NFATC4
Protein Name:Nuclear factor of activated T-cells cytoplasmic 4
Human Gene Id:4776
Human Swiss Prot No:Q14934
Mouse Gene Id:73181
Mouse Swiss Prot No:Q8K120
Immunogen:The antiserum was produced against synthesized peptide derived from human NFAT3 around the phosphorylation site of Ser676. AA range:642-691
Specificity:Phospho-NFATc4 (S676) Polyclonal Antibody detects endogenous levels of NFATc4 protein only when phosphorylated at S676.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:NFATC4;NFAT3;Nuclear factor of activated T-cells; cytoplasmic 4;NF-ATc4;NFATc4;T-cell transcription factor NFAT3;NF-AT3
Observed Band(KD):120kD
Background: This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
Function:domain:Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.,function:Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.,PTM:Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by FRAP1, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.,PTM:Ubiquitinated, leading
Subcellular Location:Cytoplasm, cytosol . Nucleus . When hyperphosphorylated, localizes in the cytosol. When intracellular Ca(2+) levels increase, dephosphorylation by calcineurin/PPP3CA leads to translocation into the nucleus (PubMed:11997522, PubMed:18347059). MAPK7/ERK5 and MTOR regulate NFATC4 nuclear export through phosphorylation at Ser-168 and Ser-170 (PubMed:18347059). .
Expression:Widely expressed, with high levels in placenta, lung, kidney, testis and ovary (PubMed:18675896). Weakly expressed in spleen and thymus (PubMed:18675896). In the hippocampus, expressed in the granular layer of the dentate gyrus, in the pyramidal neurons of CA3 region, and in the hippocampal fissure (PubMed:18675896). Expressed in the heart (at protein level) (PubMed:12370307).