Target：NBK
Fields：Endocrine resistance
Gene Name：BIK
Protein Name：Bcl-2-interacting killer
Human Gene Id：638
Human Swiss Prot No：Q13323
Mouse Swiss Prot No：O70337
Immunogen：The antiserum was produced against synthesized peptide derived from human BIK around the phosphorylation site of Thr33. AA range:18-67
Specificity：Phospho-NBK (T33) Polyclonal Antibody detects endogenous levels of NBK protein only when phosphorylated at T33.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：BIK;NBK;Bcl-2-interacting killer;Apoptosis inducer NBK;BIP1;BP4
Observed Band(KD)：30kD
Background： The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011],
Function：domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.,subcellular location:Around the nuclear envelope, and in cytoplasmic membranes.,
Subcellular Location：Endomembrane system; Single-pass membrane protein. Mitochondrion membrane ; Single-pass membrane protein . Around the nuclear envelope, and in cytoplasmic membranes.
Expression： B-cell,Lymph,Lymphoid,
