Target：MyoD
Fields：Spinocerebellar ataxia
Gene Name：MYOD1
Protein Name：Myoblast determination protein 1
Human Gene Id：4654
Human Swiss Prot No：P15172
Mouse Gene Id：17927
Mouse Swiss Prot No：P10085
Rat Gene Id：337868
Rat Swiss Prot No：Q02346
Immunogen：The antiserum was produced against synthesized peptide derived from human MYOD around the phosphorylation site of Ser200. AA range:171-220
Specificity：Phospho-MyoD (S200) Polyclonal Antibody detects endogenous levels of MyoD protein only when phosphorylated at S200.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：MYOD1;BHLHC1;MYF3;MYOD;Myoblast determination protein 1;Class C basic helix-loop-helix protein 1;bHLHc1;Myogenic factor 3;Myf-3
Observed Band(KD)：34kD
Background： This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008],
Function：function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.,online information:MyoD entry,PTM:Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.,PTM:Ubiquitinated on the N-terminus; which is required for proteasomal degradation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1.,
Subcellular Location：Nucleus.
Expression： Muscle,Skeletal muscle,
