Target：Moesin/Ezrin/Radixin
Fields：Tight junction;Leukocyte transendothelial migration;Regulation of actin cytoskeleton;Measles;Proteoglycans in cancer
Gene Name：MSN
Protein Name：Moesin
Human Gene Id：4478/5962
Human Swiss Prot No：P26038/P35241/P15311
Mouse Gene Id：17698/19684/22350
Rat Gene Id：81521/54319
Rat Swiss Prot No：O35763/P31977
Immunogen：The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin around the phosphorylation site of Thr558. AA range:524-573
Specificity：Phospho-Moesin/Ezrin/Radixin (T558) Polyclonal Antibody detects endogenous levels of Moesin/Ezrin/Radixin protein only when phosphorylated at T558.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：MSN;Moesin;Membrane-organizing extension spike protein;RDX;Radixin;EZR;VIL2;Ezrin;Cytovillin;Villin-2;p81
Observed Band(KD)：67kD
Background： Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008],
Function：function:Probably involved in connections of major cytoskeletal structures to the plasma membrane.,PTM:Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.,similarity:Contains 1 FERM domain.,subcellular location:Phosphorylated form is enriched in microvilli-like structures at apical membrane.,subunit:In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation (By similarity). Binds SLC9A3R1.,tissue specificity:In all tissues and cultured cells studied.,
Subcellular Location：Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus . Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity). Localizes at the uropods of T lymphoblasts. .
Expression：In all tissues and cultured cells studied.
