Target:Mnk1
Fields:MAPK signaling pathway;HIF-1 signaling pathway;Insulin signaling pathway
Gene Name:MKNK1
Protein Name:MAP kinase-interacting serine/threonine-protein kinase 1
Human Gene Id:8569
Human Swiss Prot No:Q9BUB5
Mouse Gene Id:17346
Mouse Swiss Prot No:O08605
Rat Gene Id:500526
Rat Swiss Prot No:Q4G050
Immunogen:The antiserum was produced against synthesized peptide derived from human MNK1 around the phosphorylation site of Thr255. AA range:221-270
Specificity:Phospho-Mnk1 (T255) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T255.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000;IHC 1:50-300
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:MKNK1;MNK1;MAP kinase-interacting serine/threonine-protein kinase 1;MAP kinase signal-integrating kinase 1;MAPK signal-integrating kinase 1;Mnk1
Observed Band(KD):60kD
Background:MAP kinase interacting serine/threonine kinase 1(MKNK1) Homo sapiens This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012],
Function:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.,function:May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.,PTM:Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.,tissue specificity:Ubiquitous.,
Subcellular Location:[Isoform 2]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus.
Expression:Ubiquitous.