Target：MLK3

Fields：MAPK signaling pathway;Non-alcoholic fatty liver disease

Gene Name：MAP3K11

Protein Name：Mitogen-activated protein kinase kinase kinase 11

Human Gene Id：4296

Human Swiss Prot No：Q16584

Mouse Gene Id：26403

Mouse Swiss Prot No：Q80XI6

Rat Gene Id：309168

Rat Swiss Prot No：Q66HA1

Immunogen：Synthesized phospho-peptide around the phosphorylation site of human MLK3 (phospho Ser674)

Specificity：Phospho-MLK3 (S674) Polyclonal Antibody detects endogenous levels of MLK3 protein only when phosphorylated at S674.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000;IHC 1:50-300

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：MAP3K11;MLK3;PTK1;SPRK;Mitogen-activated protein kinase kinase kinase 11;Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase

Molecular Weight(Da)：93kD

Background： The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP

Subcellular Location：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.

Expression：Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.