Target：PKD2
Fields：Rap1 signaling pathway;Aldosterone synthesis and secretion;Chemical carcinogenesis - reactive oxygen species
Gene Name：PRKD2
Protein Name：Serine/threonine-protein kinase D2
Human Gene Id：25865
Human Swiss Prot No：Q9BZL6
Mouse Gene Id：101540
Mouse Swiss Prot No：Q8BZ03
Rat Gene Id：292658
Rat Swiss Prot No：Q5XIS9
Immunogen：The antiserum was produced against synthesized peptide derived from human PKD2 around the phosphorylation site of Ser876. AA range:829-878
Specificity：Phospho-PKD2 (S876) Polyclonal Antibody detects endogenous levels of PKD2 protein only when phosphorylated at S876.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：PRKD2;PKD2;HSPC187;Serine/threonine-protein kinase D2;nPKC-D2
Observed Band(KD)：96kD
Background： The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.,PTM:Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Widely expressed.,
Subcellular Location：Cytoplasm . Cell membrane . Nucleus . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. .
Expression：Widely expressed.
