Target：p47-phox
Fields：Chemokine signaling pathway;Phagosome;Osteoclast differentiation;Neutrophil extracellular trap formation;Fc gamma R-mediated phagocytosis;Leukocyte transendothelial migration;Prion disease;Leishmaniasis;Chemical carcinogenesis - reactive oxygen species;Diabetic cardiomyopathy;Lipid and atherosclerosis;Fluid shear stress and atherosclerosis
Gene Name：NCF1
Protein Name：Neutrophil cytosol factor 1
Human Gene Id：653361
Human Swiss Prot No：P14598
Mouse Swiss Prot No：Q09014
Immunogen：The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser359. AA range:331-380
Specificity：Phospho-p47-phox (S359) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S359.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：NCF1;NOXO2;SH3PXD1A;Neutrophil cytosol factor 1;NCF-1;47 kDa autosomal chronic granulomatous disease protein;47 kDa neutrophil oxidase factor;NCF-47K;Neutrophil NADPH oxidase factor 1;Nox organizer 2;Nox-organizing protein 2;SH3
Observed Band(KD)：45kD
Background： The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],
Function：disease:Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).,online information:NCF1 deficiency database,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with NOXA1.,
Subcellular Location：Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side .
Expression：Detected in peripheral blood monocytes and neutrophils (at protein level).
