Target：p47-phox
Fields：Chemokine signaling pathway;Phagosome;Osteoclast differentiation;Neutrophil extracellular trap formation;Fc gamma R-mediated phagocytosis;Leukocyte transendothelial migration;Prion disease;Leishmaniasis;Chemical carcinogenesis - reactive oxygen species;Diabetic cardiomyopathy;Lipid and atherosclerosis;Fluid shear stress and atherosclerosis
Gene Name：NCF1
Protein Name：Neutrophil cytosol factor 1
Human Gene Id：653361
Human Swiss Prot No：P14598
Mouse Gene Id：17969
Mouse Swiss Prot No：Q09014
Immunogen：The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350
Specificity：Phospho-p47-phox (S328) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S328.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:10000. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：NCF1;NOXO2;SH3PXD1A;Neutrophil cytosol factor 1;NCF-1;47 kDa autosomal chronic granulomatous disease protein;47 kDa neutrophil oxidase factor;NCF-47K;Neutrophil NADPH oxidase factor 1;Nox organizer 2;Nox-organizing protein 2;SH3
Molecular Weight(Da)：45kD
Background： The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],
Function：disease:Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).,online information:NCF1 deficiency database,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with NOXA1.,
Subcellular Location：Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side .
Expression：Detected in peripheral blood monocytes and neutrophils (at protein level).
