Target：p40-phox
Fields：Phagosome;Osteoclast differentiation;Neutrophil extracellular trap formation;Leukocyte transendothelial migration;Prion disease;Leishmaniasis;Diabetic cardiomyopathy;Lipid and atherosclerosis
Gene Name：NCF4
Protein Name：Neutrophil cytosol factor 4
Human Gene Id：4689
Human Swiss Prot No：Q15080
Mouse Gene Id：17972
Mouse Swiss Prot No：P97369
Immunogen：The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154. AA range:120-169
Specificity：Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：NCF4;SH3PXD4;Neutrophil cytosol factor 4;NCF-4;Neutrophil NADPH oxidase factor 4;SH3 and PX domain-containing protein 4;p40-phox;p40phox
Molecular Weight(Da)：39kD
Background： The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding d
Function：function:Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 1 SH3 domain.,subunit:p40-PHOX associates primarily with p67-PHOX to form a complex with p47-PHOX.,tissue specificity:Expression is restricted to hematopoietic cells.,
Subcellular Location：Cytoplasm, cytosol . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane ; Peripheral membrane protein .
Expression：Expression is restricted to hematopoietic cells.
