Target：Adducin α
Gene Name：ADD1
Protein Name：Alpha-adducin
Human Gene Id：118
Human Swiss Prot No：P35611
Mouse Gene Id：11518
Mouse Swiss Prot No：Q9QYC0
Rat Gene Id：24170
Rat Swiss Prot No：Q63028
Immunogen：The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Thr445. AA range:411-460
Specificity：Phospho-Adducin α (T445) Polyclonal Antibody detects endogenous levels of Adducin α protein only when phosphorylated at T445.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：ADD1;ADDA;Alpha-adducin;Erythrocyte adducin subunit alpha
Molecular Weight(Da)：81kD
Background：adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],
Function：alternative products:Additional isoforms seem to exist,domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.,
Subcellular Location：Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Expression：Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
